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Table 5 Plasmids used in this study.

From: Genetic tools for the investigation of Roseobacter clade bacteria

Plasmids

Description

Reference

pFLP2

9.4 kb IncP Ampr Flp recombinase ori1600 oriT

[48]

pLAFR3

22.0 kb IncP Tetr RP4

[50]

pUCP20T

4.17 kb IncP Ampr Plac ori1600 oriT

[49]

pRSF1010

8.7 kb IncQ Smr Sur repA repB repC

[66]

pMMB67EH

8.8 kb IncQ Ampr lacIqPtac rrnB oriV oriT

[67]

pBBR1MCS1ab

4.72 kb Cmr lacZ Plac PT7 rep

[46]

pBBR1MCS2ab

5.14 kb Kmr lacZ Plac PT7 rep

[47]

pBBR1MCS3ab

5.23 kb Tetr lacZ Plac PT7 rep

[47]

pBBR1MCS4ab

4.95 kb Ampr lacZ Plac PT7 rep

[47]

pBBR1MCS5ab

4.77 kb Gmr lacZ Plac PT7 rep

[47]

pRhokHi-2-FbFP

7.38 kb Cm Km PT7 FbFP under control of PaphII constructed from pBBR1MCS1

[54, 55]

pEX18Ap

5.8 kb ApR, oriT+, sacB+, lacZα, suicide vector

[48]

pPS858

4.5 kb ApR, GmR, GFP+

[48]

  1. aThe derivates of the pBBR1MCS plasmid are compatible with IncQ, IncP, IncW, ColE1 and p15A ori.
  2. bDifferent derivates of pBBR1MCS were used in the different Roseobacter strains in dependence on their antibiotic susceptibilities.