Figure 2
From: Genetic tools for the investigation of Roseobacter clade bacteria
Gene knockout strategy in D. shibae DFL12T. (A) Schematic presentation of the dnr locus of D. shibae DLF12T wildtype and the corresponding Δdnr-mutant. The deletion of Dshi_3189 (dnr) after homologous recombination into the D. shibae DFL12T genome was confirmed by (B) PCR of D. shibae DFL12T (line 1) and the Δdnr knockout mutants (line 2 and 3), using the primers oPT19 and oPT22 and by (C) growth of D. shibae DFL12T and two Δdnr knockout mutants in MB supplemented with 25 mM nitrate under anaerobic conditions at 30°C and 100 rpm. Shown are the growth curves of D. shibae DFL12T (-■-), D. shibae DFL12Δdnr 1 (-□-) and D. shibae DFL12Δdnr 2 (-Δ-).