Burkholderia thailandensis harbors two identical rhl gene clusters responsible for the biosynthesis of rhamnolipids

Table 3 Primers used in this study

Primer Name	Primer Sequence (5' to 3')
rhlASVF	GCTCTAGA AGACGGTCATCCTCGTGAAC¹
rhlASVR	GGGGTACC CGGCAGCTTCGTCAGATAC¹
rhlA1PF	GGAAATGGTCGATGGGTATG²
rhlA2PF	GGCGACGGATAGCGATAAG²
rhlAR	TCGTGTACTCGTCCAGCTC
rhlATp1F	GGCGGAATTCCGGCAGGTACTGCTCCGGCCGCATCGACAGGATCTGGTCCGAGCTCGAATTAGCTTCAAA
rhlATp1R	TGCCGCGGATCATGAAGCTGTACAACTACCGGTATCTGACGAAGCTGCCGGAGCTCGAATTGGGGATCTT
rhlA5'2F	GTGGTCGTGAAAGCGGAAT
rhlA5'2R	CGGCAGCTTCGTCAGATAC
rhlA3'3F	GACCAGATCCTGTCGATGC
rhlA3'3R	CTCGATCAGCGTCATCAGC

¹ Restriction sites designed into the primers are underlined.
² Primers are constructed upward of the consensus sequence of the two promoters.

ISSN: 1471-2180