Burkholderia thailandensis harbors two identical rhl gene clusters responsible for the biosynthesis of rhamnolipids

Table 2 Maximal production and relative abundance of the HAAs and rhamnolipids produced by B. thailandensis E264

HAA/Rhamnolipid	Pseudomolecular ion	Production (mg/L)		Relative abundance (%)¹
		Glycerol	Canola oil	Glycerol	Canola oil
C₁₀-C₁₂	385	N/D²	4.59	-	-
C₁₂-C₁₂	413	N/D	N/D	-	-
C₁₂-C₁₄	441	N/D	N/D	-	-
C₁₄-C₁₄	469	N/D	N/D	-	-
C₁₄-C₁₆	497	N/D	N/D	-	-
C₁₆-C₁₆	525	1.60	7.05	-	-
Rha-C₁₀-C₁₂	531	N/D	0.98	0.00	0.07
Rha-C₁₂-C₁₂	559	0.57	6.48	0.14	0.44
Rha-C₁₂-C₁₄	587	1.86	13.75	0.45	0.94
Rha-C₁₄-C₁₄	615	24.37	94.53	5.84	6.47
Rha-C₁₄-C₁₆	643	1.16	5.42	0.28	0.37
Rha-C₁₆-C₁₆	671	N/D	N/D	0.00	0.00
Rha-Rha-C₁₀-C₁₂	677	0.75	7.44	0.18	0.51
Rha-Rha-C₁₂-C₁₂	705	7.41	49.43	1.77	3.38
Rha-Rha-C₁₂-C₁₄	733	28.48	179.73	6.82	12.29
Rha-Rha-C₁₄-C₁₄	761	321.42	1021.20	76.99	69.85
Rha-Rha-C₁₄-C₁₆	789	31.24	82.37	7.48	5.63
Rha-Rha-C₁₆-C₁₆	817	0.26	0.73	0.06	0.05
Total		419.10	1473.72

¹ Relative abundance of rhamnolipids only.
² N/D: Not detected.
Cultures were grown on 4% glycerol and canola oil as respective carbon sources. LC/MS analysis was performed after 13 days of incubation at 37°C.

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