Molecular and biochemical characterization of urease and survival of Yersinia enterocolitica biovar 1A in acidic pH in vitro

Table 3 Urease structural and accessory proteins of Y. enterocolitica biovar 1A (Ye 1A).

	Gene	Gene product (aa)	Mol. mass (Da)*	pI*	% identity/% similarity
					YeO8	YeO3	Yers	Yps	Ype	Pl	Ei	Ka
Structural subunits
UreA	ureA	100	11,048	5.29	99-100	100	97-100/100	100	100	79/95	86/95	60/82
UreB	ureB	144	15,854	9.06	84-85/85-86	85/86	84-99/85-99	86-94/88-97	78-94/79-97	60/72	61/73	36/47
UreC	ureC	572	61,026	5.64	99/100	95/97	97-99/99-100	97/99	93-97/95-99	83/91	86/94	58/73
Accessory proteins
UreE	ureE	228	25,507	6.35	86-99	NA	91-95/95-97	94/97	92-93/96-97	55/69	50/70	27/39
UreF	ureF	228	25,040	6.41	100	NA	96-99/97-100	98/99	97-98/99	67/76	65/83	22/43
UreG	ureG	221	24,181	4.94	91-100	NA	98-100/99-100	96/97	96/97	86/91	86/91	54/71
UreD	ureD	327	36,592	6.61	93-98/95-99	NA	91-98/95-99	93/96	FS	64/77	59/71	-

Comparison with different Yersinia spp. and other bacteria.
The abbreviations correspond to following species with protein accession numbers for UreA, UreB, UreC, UreE, UreF, UreG and UreD in parentheses: Ye1A: Y. enterocolitica biovar 1A (ABC74582-ABC74585; ACA51855-ACA51857); YeO8: Y. enterocolitica O8 biovar 1B (AAA50994-AAA51000, CAL11049-CAL11055); YeO3: Y. enterocolitica O3 biovar 4 (CAA79314-AA79320); Yers included Y. aldovae (AAR15084-AAR15090); Y. bercovieri (AAR15092-AAR15098); Y. frederiksenii (AAR15100-AAR15106); Y. intermedia (AAR15108-AAR15114); Y. kristensenii (AAR15117-AAR15123); Y. mollaretii (AAR15126-AAR15132); Y. rohdei (AAR15135-AAR15141); Yps: Y. pseudotuberculosis (CAH22182-CAH22176, AAA87852-AAA87858, ACA67429-ACA67435); Ype: Y. pestis (ABG14357-ABG14363; CAL21284-CAL21289; AAS62666-AAS62671; AAM84812-AAM84817; ABG17479-ABG17485; ABP39996-ABP39990; AAC78632-AAC78638); Pl: Photorhabdus luminescens (CAE14464-CAE14470); Ei: Edwardsiella ictaluri (ABD93708-ABD93706, AAT42448-AAT42445); Ka: Klebsiella aerogenes (AAA25149-AAA25154); NA: Not available; FS: frameshift mutation
* Theoretical molecular mass and pI were determined with DNASTAR

ISSN: 1471-2180