Figure 1
From: Multiple recombinants in two dengue virus, serotype-2 isolates from patients from Oaxaca, Mexico
Experimental strategy. A) The flow chart shows the experimental strategy that we followed to detect the recombinants in DENV-2 isolates. The C(91)-prM-E-NS1(2400) region from the MEX_OAX_14946_06; MEX_OAX_1020_06; MEX_OAX_739_05; MEX_OAX_1733_05; MEX_OAX_1038_05 and MEX_OAX_1656_05 isolates and the clone MEX_OAX_1656_05 were amplified and sequenced. All sequences were analyzed with RDP3 and GARD software to detect the recombinants. The analysis in silico displayed the recombinants and one parental strain. B) The E protein gene from MEX_OAX_1656_05 was cloned in TOPO TAV4 to detect possible recombinants and/or the parental sequences. One parental sequence was detected in addition to one recombinant.