Figure 7From: Pseudomonas aeruginosa vesicles associate with and are internalized by human lung epithelial cellsCF patients produce antibodies to PaAP. Purified outer membranes (OM) from S470 and vesicles (V) from S470 and S470APKO5 (KO) (2 μg) were separated by SDS-PAGE and stained with SYPRO Ruby (A) or transferred to PVDF and immunoblotted using sera from a CF patient and then reblotted with anti-PaAP (B). Molecular weight standards (kDa) and the migration of PaAP (arrow) are indicated.Back to article page