Figure 6
From: Pseudomonas aeruginosa vesicles associate with and are internalized by human lung epithelial cells
PaAP is abundant and active in vesicles from CF strains and promotes the association of P. aeruginosa vesicles with lung cells. A, Purified vesicles (approximately 10 μg) were TCA-precipitated and analyzed using SDS-PAGE and Coomassie staining. Previously identified proteins in PA01 vesicles and CF2 vesicles are indicated, and (*) highlights the lower molecular weight form of OprD found in PA01 [8]. The migration of molecular weight standards is indicated (kDa). B and C, Purified vesicles from the indicated strains (2.5 μg protein/well) were incubated (24 h, 37°C) with confluent monolayers of A549 cells (5 × 104/well) and vesicle-host cell association was compared with S470 vesicle association within each experimental set. SEM is indicated; n = 2 in triplicate.