primers for allelic disruption a | ||
---|---|---|
CAT fwd [41] | GATATAGATTGAAAAGTGGAT | F5b |
CAT rev [41] | TTATCAGTGCGACAAACTGGG | Â |
cgtfwd | atggttattgttttagtcgtgga | Â |
cgtM3 | ATCCACTTTTCAATCTATATCatatggtggatatagcggtaatg | Â |
cgtM5 | CCCAGTTTGTCGCACTGATAAttaaaaacttgcaccctttatgt | Â |
cgtrev | ctctgatcgcttcttcataaact | Â |
pmifwd | atgaaaattaaaaatatcttactgagtggg | Â |
pmiM3 | ATCCACTTTTCAATCTATATCatctaaaccattagggctttcaatatac | Â |
pmiM5 | CCCAGTTTGTCGCACTGATAActttagtgaacgaggtagaaacaaac | Â |
pmirev | ttttgtctgttaaaatcatcatcaat | Â |
lpxE fwd | atgaaaaaattcttatttaaacaaaaattttgtgaaagc | Â |
lpxEM3 | ATCCACTTTTCAATCTATATCcccaaacgctgatcgttgat | Â |
lpxEM5 | CCCAGTTTGTCGCACTGATAAcgagcgcccttatggag | Â |
lpxErev | ttaaggctttttggggcttgtaaa | Â |
eptAfwd | ttggcatcattattccatctgaggt | Â |
eptAM3 | ATCCACTTTTCAATCTATATCgcaacaccccaaaaacaacgata | Â |
eptAM5 | CCCAGTTTGTCGCACTGATAAagcctgattaacgcctatgaca | Â |
eptArev | ttactcttttttgtgtttaagcagatctaaagaa | Â |
additional primers for confirmation of gene disruption | ||
G27_951fwd | agtgattcaagatggcgtgaaaa | F1 |
G27_953rev | ccaagctcaatcatttctttgtcttt | R1 |
G27_37fwd | cggcatggggatcaatcaag | F2 |
G27_39rev | ctcccgtcttgcccggtaac | R2 |
G2719fwd | gggcgataaaatcgtgtttca | F3 |
G2721rev | tcccctttatcgtttatgctaatga | R3 |
G2720fwd | cccaaactgagcgctaaca | F4 |
G2722rev | aagaaatttcaaggtataatagtttccaag | R4 |