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Table 7 Detection parameters for selected A. niger secondary metabolites

From: Proteome analysis of Aspergillus niger: Lactate added in starch-containing medium can increase production of the mycotoxin fumonisin B2by modifying acetyl-CoA metabolism

Metabolite

 

Detection

Confirmation

  

Method1

Rt2

Std.

MS ions and adducts1

UV peak absorption wavelengths3

Fumonisin B2

[6]

MS

[M+H]+ = m/z 706

9.6

×

[M+Na]+ = m/z 728

End4

Fumonisin B4

[24]

MS

[M+H]+ = m/z 690

10.5

-

-

End4

Ochratoxin A

[5]

FLD

Excitation: 333 nm, emission: 460 nm

10.3

×

-

216 nm (100), 250 nm (sh), 332 nm (20) [69]

Ochratoxin alpha

[70]

FLD

Excitation: 333 nm, emission: 460 nm

7.1

×

-

216 nm (100), 235 nm (sh), 248 nm (sh), 336 nm (22) [69]

Malformin A1

[71]

MS

[M+NH3]+ = m/z 547

10.5

×

[M+H]+ = m/z 530, [M+Na]+ = m/z 552

End4

Malformin C

[72]

MS

[M+NH3]+ = m/z 547

10.9

×

[M+H]+ = m/z 530, [M+Na]+ = m/z 552

End4

Orlandin

[73]

MS

[M+H]+ = m/z 411

7.5

-

[M+Na]+ = m/z 433

Similar to kotanin

Desmethyl-kotanin

[30]

MS

[M+H]+ = m/z 425

9.3

-

[M+Na]+ = m/z 447

Similar to kotanin

Kotanin

[30]

MS

[M+H]+ = m/z 439

11.4

×

[M+Na]+ = m/z 461

208 nm (100), 235 nm (sh), 296 nm (sh), 308 nm (47), 316 nm (sh) [69]

Aurasperone B

[74]

MS

[M+H]+ = m/z 607

11.5

-

[M+Na]+ = m/z 629

233 nm (68), 270 nm (sh), 280 nm (100), 318 nm (24), 331 nm (24), 404 nm (15)[75]

Pyranonigrin A

[76]

MS

[M+H]+ = m/z 224

1.7

-

[M+NH4]+ = m/z 241, [M+Na]+ = m/z 246

210 nm (100), 250 nm (51), 314 nm (68) [77]

Tensidol B

[78]

MS

[M+H]+ = m/z 344

9.1

-

[M+Na]+ = m/z 366

206 nm (100), 242 nm (44) [78]

  1. List of secondary metabolites included in this study with reference of their production in A. niger. Detection method and retention time, available standards used for confirmation (marked by x) and additional MS and UV spectral information used for confirmation.
  2. 1) Values obtained from Antibase 2007 (Wiley, Hoboken, New jersey, USA).
  3. 2) Retention time in respective LC systems (OTA and OT-alpha analysis on separate HPLC system).
  4. 3) Parenthesis values are absorption in percent of maximum absorption, sh denotes a shoulder.
  5. 4) End: End absorption (< 200 nm).