Figure 1
Expression of a transcriptional pitA-lacZ fusion construct in M. smegmatis. Wild-type M. smegmatis harbouring the pitA-lacZ construct pAH1 was grown in ST medium containing 100 mM phosphate (Control), followed by 2 h starvation in phosphate-free (-Pi) or Mg2+-free (-Mg2+) ST medium, or 2 h exposure to 5 mM EDTA (+ EDTA), pH 4 or pH 9. β-Galactosidase (β-Gal) activities were assayed and are expressed in Miller Units (MU). Results are the mean ± standard deviation of three independent experiments.