Skip to main content

Advertisement

Table 2 Primer information for amplification of the fliY and bla genes.

From: Inactivation of the fliY gene encoding a flagellar motor switch protein attenuates mobility and virulence of Leptospira interrogansstrain Lai

Gene Primer sequence (5'-3') Product size
fliY F: GCC GGA TCC (BamH I) ATG GGT GAA GGT TCC CTA TCA CAG 1065 bp
  R: GCC AAG CTT (Hind III) TCA CTT ACC CTC CGG CTT AAT CCG  
bla F: GCC AGA TCT (Bgl II) TCT AAA TAC ATT CAA ATA TGT 954 bp
  R: GCC AGA TCT (Bgl II) CTT GGT CTG ACA GTT ACC AAT  
fliP F: ATG AAA ATG AGA CAT AAA 804 bp
  R: TCA TTT ATA ACT CCT TAC  
fliQ F: ATG ACG GAA TTA GAC GTT ATG 264 bp
  R: CTA AAA TTT TTC GAT CAT CAA  
  1. F: forward primer, R: reverse primer.