Table 2 Primer information for amplification of the fliY and bla genes.
Gene | Primer sequence (5'-3') | Product size |
|---|---|---|
fliY | F: GCC GGA TCC (BamH I) ATG GGT GAA GGT TCC CTA TCA CAG | 1065 bp |
| Â | R: GCC AAG CTT (Hind III) TCA CTT ACC CTC CGG CTT AAT CCG | Â |
bla | F: GCC AGA TCT (Bgl II) TCT AAA TAC ATT CAA ATA TGT | 954 bp |
| Â | R: GCC AGA TCT (Bgl II) CTT GGT CTG ACA GTT ACC AAT | Â |
fliP | F: ATG AAA ATG AGA CAT AAA | 804 bp |
| Â | R: TCA TTT ATA ACT CCT TAC | Â |
fliQ | F: ATG ACG GAA TTA GAC GTT ATG | 264 bp |
| Â | R: CTA AAA TTT TTC GAT CAT CAA | Â |