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Figure 7 | BMC Microbiology

Figure 7

From: Inactivation of the fliY gene encoding a flagellar motor switch protein attenuates mobility and virulence of Leptospira interrogansstrain Lai

Figure 7

Apoptosis ratios of J774A.1 cells induced by the fliY- mutant and wild-type strain. Panel A: lower left quadrants indicate unstained normal cells; lower right quadrants, the early apoptotic cells binding Annexin-V; upper left quadrants, the necrotic cells binding PI; and upper right quadrants, the late apoptotic/necrotic cells binding both Annexin-V and PI. Cell death was measured by flow cytometry as described in Methods. Panel B: proportion of early apoptotic cells (annexin-V+/PI-) after infection for different times. The data are expressed as mean ± SD for three independent experiments. Panel C: proportion of late apoptotic/necrotic cells (annexin-V+/PI+) after infection for different times. The data are expressed as mean ± SD for three independent experiments. *:P < 0.05, wild-type strain compared with the mutant.

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