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Figure 4 | BMC Microbiology

Figure 4

From: Use of colony-based bacterial strain typing for tracking the fate of Lactobacillusstrains during human consumption

Figure 4

Reproducibility of single colony RAPD fingerprints. The polymorphismsamplified by primer 272 from conventionally extracted DNA compared to single colony Chelex® extracted DNA are shown for two LAB strains as follows: lane 1, L. rhamnosus strain MW standard DNA extraction; lanes 2 to 4, single colonies of strain MW that were picked into Chelex® resin, stored frozen and then extracted immediately prior to PCR; lane 5, L. acidophilus strain LMG 8151 standard DNA extraction; lanes 6 to 8, single colonies of strain LMG 8151 that were processed with Chelex® as described. The size of relevant molecular size markers (lane M) are shown in bp.

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