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Table 1 Relative abundance (%) of selected bacterial groups in samples from a shelf life experiment of cod loins by cultivation, 16S rRNA clone analysis and t-RFLP.

From: Bacterial composition and succession during storage of North-Atlantic cod (Gadus morhua)at superchilled temperatures

Storage conditions

Cultivation

16S

t-RFLP1

Atmosphere (salt group)

Temp. (°C)

Time (days)

P.s.2

P.p 3

P.p 3

P.p 3

Air (LS)

-

0

7.9

Nd

0.0

0.0

Air (LS)

0

6

24.5

4.7

91.3

100

Air (LS)

0

13

28.5

6.2

95.2

84.1

Air (LS)

-2

6

29.9

2.5

61.4

40.7

Air (LS)

-2

15

58.9

1.3

93.3

86.2

Air (LS)

-4

15

42.7

Nd

83.3

100

Air (HS)

-2

6

14.0

0.5

60.0

72.3

Air (HS)

-2

15

4.8

0.7

87.8

77.1

Air (HS)

-4

15

73.3

0.03

86.0

73.1

MAP (LS)

0

7

1.2

1.7

97.4

85.5

MAP (LS)

0

15

0.02

> 99

FP

FP

MAP (LS)

0

21

0.03

21.3

100

95.1

MAP (LS)

-2

7

> 99

0.5

100

FP

MAP (LS)

-2

28

0.6

0.4

100

91.9

MAP (LS)

-4

7

34.3

Nd

100

Nd

MAP (LS)

-4

21

3.2

0.4

100

90.0

MAP (HS)

-2

13

1.4

0.1

100

94.2

MAP (HS)

-2

21

6.2

0.8

95.2

62.7

MAP (HS)

-4

7

33.5

0.04

52.5

Nd

MAP (HS)

-4

28

19.3

0.1

91.3

64.7

  1. 1Abundancy was calculated by dividing the peak area of the P. phosphoreum peak by the total peak area in the t-RFLP profile. The data was generated from analysis of reverse labelled Tf and digestion with AluI.
  2. 2Pseudomonas spp.
  3. 3 Photobacterium phosphoreum
  4. Nd, not detected
  5. FP, No PCR product