Table 3 Primers used to map Tn4371-like ICEs
From: Novel Tn4371-ICE like element in Ralstonia pickettiiand Genome mining for comparative elements
Genes | Size (bp) | Primers | Tm(°C) | R. pickettii12J Position | Accession no. | |
|---|---|---|---|---|---|---|
| Â | Â | Â | Â | Start | Stop | Â |
CirIm | ~220 | RE1 GCATGGAAGACTTGACAG LE1 GAGCTTGAGTTTTGCCACG | 54 | N\A | N\A | FM244490 |
int | 1035 | intFor1 TTTCATTTCACCATGACTCCAG intRev1 GAGAGCAGTCGATAGGCTTCC | 61.7 | 2715201 | 2716235 | FM244486 |
RepA, ParA ParB | 1657 | RepAF GAGACTACCAGCGCCTCAAG RepAR ACGTGTTCATGAGGACTTCTCC | 55 | 2734598 | 2736255 | FM244487 |
traG | 1483 | traGF GTTCGAGTGGTGGTTCTTCTTC traGR GAAATTGCTGTCCGCGTAGTAG | 61 | 2757179 | 2758661 | FM244488 |
trbI | 1597 | trbIF AACTGACCATGAGCCAGGAC trbIR AAAGCTCCTCAAAAGCGAAAG | 62 | 2767516 | 2769113 | FM244489 |