Use of nfsB, encoding nitroreductase, as a reporter gene to determine the mutational spectrum of spontaneous mutations in Neisseria gonorrhoeae

Table 4 Analysis of mutations resulting in nitrofurantoin resistance

Point mutations^a				Frameshift mutation
Nonsense		Missense		Insertions (single site)		Deletions (single site)
CAA->TAA	7	Transitions		Single base	22	Single base	16
CAG->TAG	11	C->T	5	Multiple bases	4	Multiple bases	9
TCG->TAG	9	T->C	2
GAG->TAG	5	A->G	0
TGG->TGA	1	G->A	1
		Transversions			Mutations in promoter region			3
		T->A	3
		A->T	0
		G->C	1
		C->G	1
		T->G	5
		G->T	0
		A->C	0
		C->A	2
Total:	33		20		26		25	3

^aOf the 53 point mutations examined, 27 were transitions and 26 were transversions.

ISSN: 1471-2180