Table 2 Oligonucleotides used for screening T-DNA insertion pools

T-DNA primers

   RB3

CGAATTCGAGCTCGGTACAGTGAC

58°C

   RB6

GATTGTCGTTTCCCGCCTTCAG

59°C

   LB6

TGTTGGACTGACGCAACGACCTTGTCAACC

69°C

   LB8

CAGGGACTGAGGGACCTCAGCAGGTCG

68°C

Gene-specific primers

   AGS1-50

ATCCATCATTCAACGTCCGGTA

56°C

   AGS1-72

TTGCGTACTGGGTGAGATGG

54°C

   CBP1-21

AATCACGTGGTCGCTAAATGG

54°C

   CBP1-23

CCACAAGCAGCCCTTGCATGCCTCA

67°C