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Figure 2 | BMC Microbiology

Figure 2

From: Reverse genetics through random mutagenesis in Histoplasma capsulatum

Figure 2

Gradual freezing in DMSO maximizes recovery of cryopreserved Histoplasma yeast. WU15 yeast were frozen in varying concentrations of glycerol (A) or DMSO (B). Histoplasma yeast were grown to late log/early stationary phase in rich medium and added to the appropriate glycerol- or DMSO-containing solutions before freezing. Final cryoprotectant concentrations indicated along the x-axis of each graph. Vials were placed immediately at -80°C (rapid freeze) or were placed into a styrofoam container before placement at -80°C (slow freeze). Frozen cell aliquots were thawed after 1 week or 9 weeks and recovery measured as the number of viable cfu relative to the number present before freezing.

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