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Figure 1 | BMC Microbiology

Figure 1

From: Reverse genetics through random mutagenesis in Histoplasma capsulatum

Figure 1

Sensitivity of PCR to detect individual strains within pools of Histoplasma mutants. Within a collection of Histoplasma yeast, PCR can identify cells comprising as little as 1/800th of the population. (A) Schematic representation of the nested PCR screening approach for identification of T-DNA insertions in a targeted gene. Primers specific for the T-DNA left border (LB) or right border (RB) bind within the T-DNA element and gene specific primers (GSPs) anchor PCR from the chromosome. (B) Results of primary PCR experiments to detect the OSU4-specific T-DNA insertion. Template nucleic acid from OSU4 was diluted into TE buffer (1:200, 1:800, or 1:3200 dilutions) or template nucleic acid was prepared from suspensions of OSU4 yeast mixed with random T-DNA mutants at ratios of 1:200, 1:800, or 1:3200. Negative template controls consisted of wild-type Histoplasma DNA or nucleic acid prepared from the mutant pool before spiking with OSU4 yeast. Thirty cycles of PCR were performed using RB6 and AGS1-50 primers. The approximately 1250 bp amplicon is specific for the T-DNA insertion carried by the OSU4 strain. (C) Results of nested PCR performed on dilutions of the primary PCR from (B). 1:1000, 1:10,000, and 1:100,000 serial dilutions of the primary PCR reactions were used as templates for PCR with the nested primers RB6 and AGS1-72. PCR products were separated by electrophoresis through 1% agarose.

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