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Table 2 Accurate identification rate achieved with different approaches to interpretation of McRAPD data.

From: Performance of optimized McRAPD in identification of 9 yeast species frequently isolated from patient samples: potential for automation

Species

N

Normalized curves

Normalized curves + matching of derivative peaks

Visual matching of derivative plots

Matching of RAPD fingerprints

Candida albicans

44

63.6

72.7

100

100

Candida glabrata

41

58.5

82.9

97.6

97.6

Candida krusei

39

64.1

82.1

97.4

100

Candida tropicalis

40

100.0

97.5

100

100

Saccharomyces cerevisiae

39

89.7

92.3

100

100

Candida parapsilosis

38

73.7

78.9

100

100

Candida lusitaniae

41

97.6

97.6

100

100

Candida guilliermondii

19

94.7

94.7

94.7

94.7

Candida pelliculosa

17

88.2

82.4

82.4-88.2

100

Candida metapsilosis

4

75.0

100.0

100

100

All species studied

322

79.5

86.7

98.1-98.4

99.4

  1. Normalized curves column stays for accurate identification rate achieved when identification was based on automated determination of the numerically closest match of the examined curve with known strain. Normalized curve + matching of derivative peaks column stays for the same amended by checking for decisive peaks in derivative plot. Visual matching of derivative plots column stays for accurate identification rate achieved when identification was based on simple visual comparison of examined derivative plot with plots of known strains. Accurate identification rate achieved upon evaluation and matching of RAPD fingerprints is shown for reference in the last column. See Results and discussion for details.