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Figure 3 | BMC Microbiology

Figure 3

From: Performance of optimized McRAPD in identification of 9 yeast species frequently isolated from patient samples: potential for automation

Figure 3

Reproducibility of McRAPD with crude colony lysates sampled from different colonies at different timepoints. DNA extraction was performed in 4 different yeast species, each represented by one strain, where 5 colonies were subcultured for different time periods in each strain. Part (A) normalized melting curves, part (B) derivative curves, part (C) and (D) fingerprints after agarose gel electrophoresis with the 200-1500 molecular weight marker (Top-Bio, Prague, Czech Republic) in lanes 1 and 12. Dark green lines and lanes 2-6 in part (C) C. tropicalis I3-CATR9-17; light green lines and lanes 7-11 in part (C) C. krusei I1-CAKR-06; violet lines and lanes 2-6 in part (D) C. pelliculosa I3-CAPE3-04; and blue lines and lanes 7-11 in part (D) C. guilliermondii I1-CAGU-22.

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