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Figure 2 | BMC Microbiology

Figure 2

From: Performance of optimized McRAPD in identification of 9 yeast species frequently isolated from patient samples: potential for automation

Figure 2

Comparison of McRAPD results obtained with DNA extracted using the commercial kit YeaStar Genomic DNA Kit ( Zymo Research, Orange, CA, USA ) and using the technique of crude colony lysates. Selected strains were subjected to DNA extraction in parallel and the DNA was used for McRAPD resulting in duplicates of melting curves and duplicates of agarose gel fingerprints. In each duplicate solid lines in plots and left lanes in gel represent results obtained with the DNA extracted using the commercial kit, whereas dotted lines and right lanes represent results obtained with 1 μl of crude colony lysate. Part (A): normalized melting curves, part (B) derivative curves, part (C) fingerprints obtained with agarose gel electrophoresis, lane 1 and 20 molecular weight marker 200-1500 (Top-Bio, Prague, Czech Republic). Lane 2, 3 and black line C. lusitaniae I1-CALU-33, lane 4, 5 and violet line C. guilliermondii I1-CAGU2-20, lane 6, 7 and blue line C. pelliculosa I3-CAPE3-10, lane 8, 9 and yellow line S. cerevisiae I3-SACE3-37, lane 10, 11 and orange line C. metapsilosis I1-CAME7-11, lane 12,13 and dark green line C. tropicalis I3-CATR9-22, lane 14, 15 and light green line C. krusei I1-CAKR-24, lane 16, 17 and turquoise line C. glabrata I1-CAGL-39, lane 18, 19 and red line C. albicans ATCC 76615.

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