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Table 1 Reverse primers used for the construction of plasmid pHKU1601 series.

From: Topological analysis of a haloacid permease of a Burkholderi a sp. bacterium with a PhoA-LacZ reporter

Plasmid Primer Sequence (5' to 3')
pHKU1601-034 4p034R-speI ACTAG TGTCA TACCA CTCGA ATACG GTTCC CAA
pHKU1601-052 4p052R-speI ACTAG TACCG GAGAA GAACG TTCGG CT
pHKU1601-062 4p062R-speI ACTAG TTGTG AACAC AAACC CCGCT GCTG
pHKU1601-076 4p076R-speI ACTAG TGCCA AAAGG ACGCA CGGCG
pHKU1601-090 4p090R-speI ACTAG TCTTG CGTCC GATCA TGTCT CCAAG
pHKU1601-100 4p100R-speI ACTAG TCATC AGCAG GATTG TCGCA AGAA
pHKU1601-114 4p114R-speI ACTAG TTCCG TAACC GGGCA ACAAT CCAA
pHKU1601-126 4p126R-speI ACTAG TAGCG ATGAA AACAA CCGGC GC
pHKU1601-138 4p138R-speI ACTAG TCTCT CCGCC AAGCG CCAG
pHKU1601-150 4p150R-speI ACTAG TTGCG TGTTC CGCAA CATAG GTC
pHKU1601-163 4p163R-speI ACTAG TCTGG ATCCA TGCGG TCCAT GCG
pHKU1601-172 4p172R-speI ACTAG TAATA AACAG GCCAA GCGTA GCCGT
pHKU1601-191 4p191R-speI ACTAG TGGCC GCAAA TGTAT CTTCG TTAAG CAA
pHKU1601-203 4p203R-speI ACTAG TAACG ATCGA GACAA GGAAA GGAAC G
pHKU1601-220 4p220R-speI ACTAG TAACG GGTGA CTCGT GAAGT TGC
pHKU1601-240 4p240R-speI ACTAG TCCCG AATGC TTCCG ATAGT GGGG
pHKU1601-252 4p252R-speI ACTAG TTAGT GCAAG CAGGA CGATT TTCAG
pHKU1601-266 4p266R-speI ACTAG TGCCC GTGTA CCATA CAACC GCC
pHKU1601-284 4p284R-speI ACTAG TGCTC GTACC GTCGA CCTTA AGAGT CTG
pHKU1601-296 4p296R-speI ACTAG TACCG ATCAG CAACG CGACA G
pHKU1601-313 4p313R-speI ACTAG TCTTT CGCCC AATCT TGTCC GACAG
pHKU1601-322 4p322R-speI ACTAG TAATC AGGCA GCCTG CCATG ATA
pHKU1601-337 4p337R-speI ACTAG TGTAG TGGGC GAGAG CCTTG AAC
pHKU1601-358 4p358R-speI ACTAG TGCTC GGATC AGCGA TCATC G
pHKU1601-379 4p379R-speI ACTAG TTGCG ACGTC ACACG AACTC G
pHKU1601-400 4p400R-speI ACTAG TGACA GTCCC GGCAG GGGC
pHKU1601-421 4p421R-speI ACTAG TTTTT GCGTC CGCCG CTTTC
pHKU1601-442 4p442R-speI ACTAG TGGCG GGGTA GCCAG CAGTC T
pHKU1601-454 4p454R-speI ACTAG TCGAC ATCGG CCAGT TGATC AGCG
pHKU1601-466 4p466R-speI ACTAG TGGTG ACGTA GAGCA CGAGT ATCGT CAG
pHKU1601-479 4p479R-speI ACTAG TCATC TCCAC CAGCA TTGCT GCG
pHKU1601-493 4p493R-speI ACTAG TATAA GGCAG CGACA TTGAG GTGTA TCG
pHKU1601-502 4p502R-speI ACTAG TGCCG CCGAA CCAGC CATTG
pHKU1601-520 4p520R-speI ACTAG TTGAA TAGAT GTTCC CGCGC GCTG
pHKU1601-532 4p532R-speI ACTAG TCGCA ACGGA AGCGA TAACA ATC
pHKU1601-552 4p552R-speI ACTAG TGTCC GCGTC ATAGG TAGAA GAACC CTT