Analysis of gpx3 mRNA in cells exposed to heat shock and cadmium stress. A-Northern blot assay was performed using total RNA extracted from B. emersonii cells submitted to different cadmium concentrations or to heat shock. RNA extracted from cells 60 min after sporulation induction (lane 1). RNA extracted from cells submitted to heat shock (38°C) from 30 to 60 min (lane 2) after induction of sporulation. RNA extracted from cells 60 min after sporulation induction, incubated with 50 μM or 100 μM CdCl2 from 30 to 60 min (lanes 3 and 4, respectively) after sporulation induction. As a control of RNA levels, the 28S rRNA was shown. B -- Relative transcript levels of gpx3 mRNA determined by densitometry scanning of the autoradiogram shown in A. The figure legend (1, 2, 3 and 4) is the same depicted above.