Figure 3

Splicing of hsp70 mRNA is inhibited in B. emersonii cells exposed to cadmium. Nuclease Sl protection assays were performed using the 5' end-labeled probe, depicted above the autoradiogram, and 50 μg of yeast tRNA in the presence or absence of nuclease S1 (lanes 1 and 2) as control, 50 μg of total RNA isolated from 60 min sporulating cells (lane 3), cells submitted to heat shock at 38°C and 42°C for 30 min (lanes 4 and 5, respectively), cells submitted to 250 μM CdCl₂ for 30 min and 60 min (lanes 6 and 7, respectively), cells submitted to 500 μM H₂O₂ for 30 min and 60 min (lanes 8 and 9, respectively).