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Table 5 Confirmed isolates of Cronobacter spp. by biochemical testing (API 20E), chromogenic (α-MUG, DFI and EsPM), eight sets of Cronobacter spp. specific primers (α-GluA, α-GluB, SG, SI, Saka, OmpA, zpx and BAM) and 16S rRNA sequence analysis.

From: Isolation of Cronobacter spp. (formerly Enterobacter sakazakii) from infant food, herbs and environmental samples and the subsequent identification and confirmation of the isolates using biochemical, chromogenic assays, PCR and 16S rRNA sequencing

Isolate      PCR Primers  
ID Source API 20E α-MUG DFI EsPM$ α-GluA α-GluB SG SI Saka OmpA zpx BAM 16S rRNA
51329 ATCC + + + BB + ND§ + + + + + +* Crono. £
29544 ATCC + + + BB + + ND ND ND ND + + Crono.
Jor32 Infant food + + + BB + ND + + + + + + Crono.
Jor20B Spices + + + BB + ND + + + + + + Crono.
Jor22 Chamomile + + + BB + ND + + + + + + Crono.
Jor44A Spices + + + BB + ND + + + + - + Crono.
Jor44B Spices + + + BB + ND + + + + + + Crono.
Jor77 Anise + + + BB + ND + + + + + +* Crono.
Jor93 spices + + + BB + ND + + + + - + Crono.
Jor95 Anise + + + BB + ND + + + + + +* Crono.
Jor96 Fennel + + + BB + ND + + + + - + Crono.
Jor112 Liquorice + + + BB + ND + + + + + +* Crono.
Jor146B Liquorice + + + BB + ND + + + + - + Crono.
Jor148 Spices + + + BB + ND + + + + + + Crono.
Jor149 Anise + + + BB - - + + + + + +* Crono.
Jor154 Spices + + + BB - + + - + + + + Crono.
Jor160A Vac dust¥ + + + BB + ND + + + + + + Crono.
Jor160B Vac dust¥ + + + BB + ND + + + + - + Crono.
Jor171 Fennel + + + BB + ND + + + + + +* Crono.
Jor172 Spices + + + BB + ND + + - + + + Crono.
Jor173 Spices + + + BB + ND + + + + + + Crono.
Jor174 Anise + + + BB + ND + + + + - +* Crono.
Jor175 Spices + + + BB - - + + + + + + Crono.
Jor176 Thyme + + + BB + ND + + - - - +* Crono.
Jor183 Spices + + + BB + ND + + + + + + Crono.
Jor204 Liquorice + + + BB + - + + + + + + Crono.
Jor146A Liquorice + + + BB + ND + + + + + + Crono.
Jor178 Chamomile + + + BB + ND + + + + - + Crono.
Jor52 Sage + + + Y/Gr - ND - - - - - -*# Crono.
Jor170 Fennel + + + Gray - ND - - - + - - Crono.
Jor184 Spices + + + Y/Gr## - ND + + + + + - Crono.
Total +   31 31 31 28 25 2 28 27 26 28 21 28  
  1. $On EsPM, colonies were blue black (BB) in chromogenic reaction color within 24 h at 37°C. The PCR conditions for BAM primers as described in Table 1 were used for amplification of both regions of the zpx gene as described by Kothary et al. [13]. Analysis of the Cronobacter and non-Cronobacter strains was performed in a similar fashion. ¥ Vacuum dust. ND§: not determined. * Multiple bands. *#, PCR product was approximately (400 bp) and sequence was found not to be zpx. ##Colonies were blue black (BB) after three days at 37°C. £ Crono; Cronobacter spp.