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Table 5 Confirmed isolates of Cronobacter spp. by biochemical testing (API 20E), chromogenic (α-MUG, DFI and EsPM), eight sets of Cronobacter spp. specific primers (α-GluA, α-GluB, SG, SI, Saka, OmpA, zpx and BAM) and 16S rRNA sequence analysis.

From: Isolation of Cronobacter spp. (formerly Enterobacter sakazakii) from infant food, herbs and environmental samples and the subsequent identification and confirmation of the isolates using biochemical, chromogenic assays, PCR and 16S rRNA sequencing

Isolate

    

PCR Primers

 

ID

Source

API 20E

α-MUG

DFI

EsPM$

α-GluA

α-GluB

SG

SI

Saka

OmpA

zpx

BAM€

16S rRNA

51329

ATCC

+

+

+

BB

+

ND§

+

+

+

+

+

+*

Crono. £

29544

ATCC

+

+

+

BB

+

+

ND

ND

ND

ND

+

+

Crono.

Jor32

Infant food

+

+

+

BB

+

ND

+

+

+

+

+

+

Crono.

Jor20B

Spices

+

+

+

BB

+

ND

+

+

+

+

+

+

Crono.

Jor22

Chamomile

+

+

+

BB

+

ND

+

+

+

+

+

+

Crono.

Jor44A

Spices

+

+

+

BB

+

ND

+

+

+

+

-

+

Crono.

Jor44B

Spices

+

+

+

BB

+

ND

+

+

+

+

+

+

Crono.

Jor77

Anise

+

+

+

BB

+

ND

+

+

+

+

+

+*

Crono.

Jor93

spices

+

+

+

BB

+

ND

+

+

+

+

-

+

Crono.

Jor95

Anise

+

+

+

BB

+

ND

+

+

+

+

+

+*

Crono.

Jor96

Fennel

+

+

+

BB

+

ND

+

+

+

+

-

+

Crono.

Jor112

Liquorice

+

+

+

BB

+

ND

+

+

+

+

+

+*

Crono.

Jor146B

Liquorice

+

+

+

BB

+

ND

+

+

+

+

-

+

Crono.

Jor148

Spices

+

+

+

BB

+

ND

+

+

+

+

+

+

Crono.

Jor149

Anise

+

+

+

BB

-

-

+

+

+

+

+

+*

Crono.

Jor154

Spices

+

+

+

BB

-

+

+

-

+

+

+

+

Crono.

Jor160A

Vac dust¥

+

+

+

BB

+

ND

+

+

+

+

+

+

Crono.

Jor160B

Vac dust¥

+

+

+

BB

+

ND

+

+

+

+

-

+

Crono.

Jor171

Fennel

+

+

+

BB

+

ND

+

+

+

+

+

+*

Crono.

Jor172

Spices

+

+

+

BB

+

ND

+

+

-

+

+

+

Crono.

Jor173

Spices

+

+

+

BB

+

ND

+

+

+

+

+

+

Crono.

Jor174

Anise

+

+

+

BB

+

ND

+

+

+

+

-

+*

Crono.

Jor175

Spices

+

+

+

BB

-

-

+

+

+

+

+

+

Crono.

Jor176

Thyme

+

+

+

BB

+

ND

+

+

-

-

-

+*

Crono.

Jor183

Spices

+

+

+

BB

+

ND

+

+

+

+

+

+

Crono.

Jor204

Liquorice

+

+

+

BB

+

-

+

+

+

+

+

+

Crono.

Jor146A

Liquorice

+

+

+

BB

+

ND

+

+

+

+

+

+

Crono.

Jor178

Chamomile

+

+

+

BB

+

ND

+

+

+

+

-

+

Crono.

Jor52

Sage

+

+

+

Y/Gr

-

ND

-

-

-

-

-

-*#

Crono.

Jor170

Fennel

+

+

+

Gray

-

ND

-

-

-

+

-

-

Crono.

Jor184

Spices

+

+

+

Y/Gr##

-

ND

+

+

+

+

+

-

Crono.

Total +

 

31

31

31

28

25

2

28

27

26

28

21

28

 
  1. $On EsPM, colonies were blue black (BB) in chromogenic reaction color within 24 h at 37°C. € The PCR conditions for BAM primers as described in Table 1 were used for amplification of both regions of the zpx gene as described by Kothary et al. [13]. Analysis of the Cronobacter and non-Cronobacter strains was performed in a similar fashion. ¥ Vacuum dust. ND§: not determined. * Multiple bands. *#, PCR product was approximately (400 bp) and sequence was found not to be zpx. ##Colonies were blue black (BB) after three days at 37°C. £ Crono; Cronobacter spp.