Table 1 Bacterial Strains and Plasmids

B. cenocepacia strains

K56-2 (LMG18863)

ET12 clone related to J2315, CF clinical isolate

[43]

JNRH1

K56-2BCAL0210::pJH9, Tp^r

This study

E. coli strains

DH5α

F^-, ϕ 80 lac ZΔM15 endA1 recA1 hsdR 17(r_K^-m_K⁺)supE 44 thi- 1 ΔgyrA 96 (ΔlacZYA-arg F)U169 relA 1

Invitrogen

SY327

araD Δ(lac pro) argE (Am) recA 56 Rif^r nalA λ pir

[40]

Plasmids

pGPΩTp

ori _r6K, ΩTp^r mob+

[27]

pRK2013

ori_colE1, RK2 derivative, Km^r mob⁺ tra⁺

[42]

pJH9

pGPΩTp, internal fragment from BCAL0210

This study

pJH1

pap20, eGFP

[9]

pJH2

pJH1, eGFP replaced promoter with multiple cloning site

This study

pJH5

pJH2, BCAL0211 promoter region (P _BCAL0211 )

This study

pJH6

pJH2, paaZ promoter region (P _paaZ )

This study

pJH7

pJH2, paaA promoter region (P _paaA )

This study

pJH8

pJH2 paaH promoter region (P _paaH )

This study

pJH10

pJH7, ACCGACCGGTCGGT → TAGATGTATCTCAG

This study

pJH11

pJH7, ACCGACCGGTCGGT → TAGATGTGGTCGGT

This study

pJH12

pJH7, ACCGACCGGTCGGT → ACCGACCATCTCAG

This study