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Table 1 Bacterial Strains and Plasmids

From: Regulation of phenylacetic acid degradation genes of Burkholderia cenocepacia K56-2

Strain or plasmid Features Reference or source
B. cenocepacia strains   
K56-2 (LMG18863) ET12 clone related to J2315, CF clinical isolate [43]
JNRH1 K56-2BCAL0210::pJH9, Tpr This study
E. coli strains   
DH5α F-, ϕ 80 lac ZΔM15 endA1 recA1 hsdR 17(rK-mK+)supE 44 thi- 1 ΔgyrA 96 (ΔlacZYA-arg F)U169 relA 1 Invitrogen
SY327 araD Δ(lac pro) argE (Am) recA 56 Rifr nalA λ pir [40]
Plasmids   
pGPΩTp ori r6K, ΩTpr mob+ [27]
pRK2013 oricolE1, RK2 derivative, Kmr mob+ tra+ [42]
pJH9 pGPΩTp, internal fragment from BCAL0210 This study
pJH1 pap20, eGFP [9]
pJH2 pJH1, eGFP replaced promoter with multiple cloning site This study
pJH5 pJH2, BCAL0211 promoter region (P BCAL0211 ) This study
pJH6 pJH2, paaZ promoter region (P paaZ ) This study
pJH7 pJH2, paaA promoter region (P paaA ) This study
pJH8 pJH2 paaH promoter region (P paaH ) This study
pJH10 pJH7, ACCGACCGGTCGGT → TAGATGTATCTCAG This study
pJH11 pJH7, ACCGACCGGTCGGT → TAGATGTGGTCGGT This study
pJH12 pJH7, ACCGACCGGTCGGT → ACCGACCATCTCAG This study
  1. Cm, chloramphenicol; Km, kanamycin; Tp, trimethoprim. Arrows represent changes introduced to indicated sequences.