Figure 6
Analyses of CDT localization in subcellular C. jejuni fractions. Subcellular localization of CDT subunits in C. jejuni strain 81-176. (A), SDS-PAGE gel after silver staining and (B), immunoblot analyses of cell fractions from C. jejuni wild type strain 81-176 (lanes 1-5) and the cdtA::km mutant (lanes 6-10). Lanes 1&6, whole cell lysates; Lanes 2&7, cytoplasmic fractions 3 & 8, inner membrane fractions; Lanes 4 & 9, periplasmic fractions; Lanes 5 & 10, outer membrane fractions. Antisera for detection of CdtA, CdtB, CdtC, CRP, and HtrA, respectively, were used for the immunoblot analyses and representative results of repeated experiments are shown. Molecular weight markers are shown in the lane (MW) on the left. See materials and methods for details about the relative amount of the extracts used.