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Figure 2 | BMC Microbiology

Figure 2

From: Surface proteins that promote adherence of Staphylococcus aureusto human desquamated nasal epithelial cells

Figure 2

Western immunoblot to detect expression of ClfB, SdrC, SdrD and SdrE. A-D. Cell wall associated proteins from strains Newman, Newman clfA, Newman clfA clfB, Newman clfA isdA clfB, Newman clfA clfB sdrCDE, Newman clfA isdA clfB sdrCDE, Newman clfA sdrCDE, Newman clfA isdA sdrCDE and Newman clfA isdA clfB sdrCDE (pCU1) and (A) Newman clfA isdA clfB sdrCDE (pCU1clfB+), (B) Newman clfA isdA clfB sdrCDE (pCU1sdrC+), (C) Newman clfA isdA clfB sdrCDE (pCU1sdrD+), (D) Newman clfA isdA clfB sdrCDE (pCU1sdrE+) were grown to the exponential phase in TSB, cell wall associated proteins were solubilised by lysostaphin and run on 7.5% SDS-PAGE gels. Western immunoblotting was performed with (A) rabbit anti-ClfB antibodies, (B) rabbit anti-SdrC antibodies, (C) rabbit anti-SdrD antibodies and (D) rabbit anti-SdrE antibodies and subsequently with HRP-conjugated protein A-peroxidase.

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