Figure 4

Compound D7 does not reduce HeLa cell viability. A: subconfluent HeLa cell monolayers incubated in MEM containing either DMSO (0.1%) or compound D7 (10 μM) with 2 μg/mL cycloheximide (+), were collected by trypsinization and the cell density was measured by absorbance at 800 nM at the times indicated. Compound D7 did not significantly alter HeLa cell number compared to DMSO alone. B: cell culture supernatant adenylate kinase activity from the samples in (A). Exposure of HeLa cells to 10 μM compound D7 for 44 hours was not more cytotoxic than cells exposed to DMSO. At 66 hours there was a small increase in HeLa cell release of adenylate kinase in the D7-exposed group. Error bars represent means plus 2 standard deviations.