Inhibition of PknD by compound D7. A: compound D7, but not compound D4 or DMSO, substantially inhibited PknD autophosphorylation (32P-GST-PknD KD) at 5 and 1 μM, as seen by autoradiography. B: related compounds D5 and D6 did not inhibit PknD at 1 or 10 μM. C: 1 mM DTT and 1% Triton X-100 did not decrease inhibition of PknD by compound D7 (used at 10 μM in all panels). DMSO (0.1%) is shown as control. D: compound D7 inhibited phosphorylation of the FHA-2 domain (32P-His-FHA-2) of CdsD by PknD. Western blotting showed equivalent amounts of protein in each autoradiograph (lower panels).