Reporter plasmids and co-integrates. Cartoon representations of the F. tularensis LVS genomic organizations of the ripA locus (a), pBSK ripA'-lacZ 2 transcriptional reporter plasmid (b), and the ripA::pBSK ripA'lacZ cointegrate (c). The ripA locus is present in only one copy in ripA::pBSK ripA'-lacZ 2 however the promoter is duplicated by the insertion resulting maintenance of the entire wild type ripA locus as well as the ripA'-lacZ reporter. The predicted ripA promoter is represented by a black arrow (a-c). pBSK ripA'-lacZ 2 is shown in gray while the alleles of the native locus are white.