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Table 1 List of phage displayed peptides and their corresponding Mmm SC proteins identified after selection with the indicated antibody subpopulations from animal C11 and the Botswana outbreak

From: Identification of genes coding for B cell antigens of Mycoplasma mycoides subsp. mycoides Small Colony (Mmm SC) by using phage display

Mmm SC protein match Identified by serum from: Accession numbera Locus Tag
  Animal C11c Botswana outbreakc   
IgG panning     
Putative variable surface protein Yes (3) ND CAE77433 MSC_0818
Cell division protein (FtsZ) Yes (1) ND CAE77211 MSC_0588
ABC transporter, substrate-binding component (Abc) Yes (1) ND CAE77420b MSC_0804
Prolipoprotein (Lpp) Yes (1) ND CAE77256 MSC_0635
D-lactate dehydrogenase (Ldh) Yes (2) ND CAE76687 MSC_0034
DNA gyrase subunit A (GyrA) Yes (2) ND CAE76660 MSC_0007
DNA gyrase subunit B (GyrB) Yes (1) ND CAE76659 MSC_0006
PTS system, glucose-specific, IIBC component (PtsG) Yes (4) ND CAE77485b MSC_0873
IgG2 panning     
Transposase ISMmy1 C Yes (1) No CAE77424 MSC_0808
30S ribosomal protein S5 (RpsE) Yes (1) No CAE77346 MSC_0728
Putative glucose-specific IIABC component (PtsG) Yes (1) No CAE76806 MSC_0161
Prolipoprotein (Lpp) Yes (1) No CAE77256 MSC_0635
Prolipoprotein LppC (LppC) Yes (1) No CAE76772 MSC_0122
Alanine-tRNA ligase (AlaS) Yes (1) No CAE76823 MSC_0178
DNA gyrase subunit B (GyrB) Yes (1) Yes (1) CAE76659 MSC_0006
Methionine adenosyltransferase (MetK) Yes (1) No CAE77120 MSC_0492
Glyceraldehyde-3-phosphate dehydrogenase (GapN) Yes (1) No CAE77137b MSC_0509
Glycerol-3-phosphate oxidase (GlpO) Yes (1) Yes (1) CAE76900b MSC_0259
D-lactate dehydrogenase (Ldh) Yes (1) No CAE76687 MSC_0034
P115-like protein with SMC_C motif Yes (7) No CAE77104 MSC_0476
Ribose/Galactose ABC transporter, ATP-binding component (RbsA) Yes (3) No CAE76663 MSC_0010
Prolipoprotein B (LppB) Yes (2) Yes (5) CAE77147b MSC_0519
Glucose inhibited division protein A (GidA) Yes (1) No CAE77646 MSC_1042
ABC transporter, substrate-binding component (Abc) Yes (4) Yes (1) CAE77420b MSC_0804
Fructose-bisphosphate aldolase class II (FbaA2) Yes (1) No CAE76786 MSC_0139
IgA panning     
Glyceraldehyde-3-phosphate dehydrogenase (NADP) (GapN) Yes (1) No CAE77137b MSC_0509
Prolipoprotein B (LppB) No Yes (3) CAE77147b MSC_0519
ABC transporter, substrate-binding component (Abc) No Yes (2) CAE77420b MSC_0804
CTP synthase (CtrA) Yes (1) No CAE76781 MSC_0134
Prolipoprotein LppC (LppC) No Yes (1) CAE76772 MSC_0122
Thioredoxin reductase (NADPH) (TrxB) Yes (1) No CAE77547 MSC_0938
Asparagine-tRNA ligase (AsnS) Yes (1) No CAE76732 MSC_0080
  1. a EMBL/GenBank accession numbers for the protein sequences.
  2. b Proteins that were expressed in this study (see Figure 3).
  3. c Figures in parenthesis indicate numbers of peptides selected
  4. ND - not determined