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Table 1 List of phage displayed peptides and their corresponding Mmm SC proteins identified after selection with the indicated antibody subpopulations from animal C11 and the Botswana outbreak

From: Identification of genes coding for B cell antigens of Mycoplasma mycoides subsp. mycoides Small Colony (Mmm SC) by using phage display

Mmm SC protein match

Identified by serum from:

Accession numbera

Locus Tag

 

Animal C11c

Botswana outbreakc

  

IgG panning

    

Putative variable surface protein

Yes (3)

ND

CAE77433

MSC_0818

Cell division protein (FtsZ)

Yes (1)

ND

CAE77211

MSC_0588

ABC transporter, substrate-binding component (Abc)

Yes (1)

ND

CAE77420b

MSC_0804

Prolipoprotein (Lpp)

Yes (1)

ND

CAE77256

MSC_0635

D-lactate dehydrogenase (Ldh)

Yes (2)

ND

CAE76687

MSC_0034

DNA gyrase subunit A (GyrA)

Yes (2)

ND

CAE76660

MSC_0007

DNA gyrase subunit B (GyrB)

Yes (1)

ND

CAE76659

MSC_0006

PTS system, glucose-specific, IIBC component (PtsG)

Yes (4)

ND

CAE77485b

MSC_0873

IgG2 panning

    

Transposase ISMmy1 C

Yes (1)

No

CAE77424

MSC_0808

30S ribosomal protein S5 (RpsE)

Yes (1)

No

CAE77346

MSC_0728

Putative glucose-specific IIABC component (PtsG)

Yes (1)

No

CAE76806

MSC_0161

Prolipoprotein (Lpp)

Yes (1)

No

CAE77256

MSC_0635

Prolipoprotein LppC (LppC)

Yes (1)

No

CAE76772

MSC_0122

Alanine-tRNA ligase (AlaS)

Yes (1)

No

CAE76823

MSC_0178

DNA gyrase subunit B (GyrB)

Yes (1)

Yes (1)

CAE76659

MSC_0006

Methionine adenosyltransferase (MetK)

Yes (1)

No

CAE77120

MSC_0492

Glyceraldehyde-3-phosphate dehydrogenase (GapN)

Yes (1)

No

CAE77137b

MSC_0509

Glycerol-3-phosphate oxidase (GlpO)

Yes (1)

Yes (1)

CAE76900b

MSC_0259

D-lactate dehydrogenase (Ldh)

Yes (1)

No

CAE76687

MSC_0034

P115-like protein with SMC_C motif

Yes (7)

No

CAE77104

MSC_0476

Ribose/Galactose ABC transporter, ATP-binding component (RbsA)

Yes (3)

No

CAE76663

MSC_0010

Prolipoprotein B (LppB)

Yes (2)

Yes (5)

CAE77147b

MSC_0519

Glucose inhibited division protein A (GidA)

Yes (1)

No

CAE77646

MSC_1042

ABC transporter, substrate-binding component (Abc)

Yes (4)

Yes (1)

CAE77420b

MSC_0804

Fructose-bisphosphate aldolase class II (FbaA2)

Yes (1)

No

CAE76786

MSC_0139

IgA panning

    

Glyceraldehyde-3-phosphate dehydrogenase (NADP) (GapN)

Yes (1)

No

CAE77137b

MSC_0509

Prolipoprotein B (LppB)

No

Yes (3)

CAE77147b

MSC_0519

ABC transporter, substrate-binding component (Abc)

No

Yes (2)

CAE77420b

MSC_0804

CTP synthase (CtrA)

Yes (1)

No

CAE76781

MSC_0134

Prolipoprotein LppC (LppC)

No

Yes (1)

CAE76772

MSC_0122

Thioredoxin reductase (NADPH) (TrxB)

Yes (1)

No

CAE77547

MSC_0938

Asparagine-tRNA ligase (AsnS)

Yes (1)

No

CAE76732

MSC_0080

  1. a EMBL/GenBank accession numbers for the protein sequences.
  2. b Proteins that were expressed in this study (see Figure 3).
  3. c Figures in parenthesis indicate numbers of peptides selected
  4. ND - not determined