Table 1 Selected TBDMSa-amino acid fragments used in the study derived from D. shibae and P. gallaeciensis grown on 99% [1-13C] glucose.
Fragment | C-Atoms | Mass isotopomer distribution (% of total pool) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
D. shibae | P. gallaeciensis | |||||||||
M +0 | M +1 | M +2 | M +3 | M +0 | M +1 | M +2 | M +3 | |||
Ala | M-57 | 1-3 | 50.0 ± 0.2 | 48.2 ± 0.2 | 1.8 ± 0.0 | 0.01 ± 0.01 | 49.2 ± 0.0 | 49.3 ± 0.0 | 1.5 ± 0.0 | 0.0 ± 0.0 |
M-85 | 2-3 | 96.8 ± 0.1 | 3.2 ± 0.1 | 0.0 ± 0.0 | 97.2 ± 0.0 | 2.8 ± 0.0 | 0.0 ± 0.0 | |||
f302 | 1-2 | 51.2 ± 0.1 | 48.2 ± 0.1 | 0.6 ± 0.0 | 50.1 ± 0.1 | 49.3 ± 0.1 | 0.6 ± 0.0 | |||
Asp | M-57 | 1-4 | 72.4 ± 0.7 | 23.2 ± 0.5 | 4.3 ± 0.2 | 0.12 ± 0.01 | 64.2 ± 0.2 | 29.4 ± 0.1 | 6.2 ± 0.2 | 0.13 ± 0.07 |
M-85 | 2-4 | 83.3 ± 0.6 | 16.2 ± 0.6 | 0.4 ± 0.1 | 0.10 ± 0.03 | 80.0 ± 0.1 | 19.4 ± 0.0 | 0.6 ± 0.0 | 0.04 ± 0.02 | |
f302 | 1-2 | 82.1 ± 0.3 | 17.6 ± 0.3 | 0.2 ± 0.0 | 76.3 ± 0.1 | 23.5 ± 0.0 | 0.3 ± 0.1 | |||
Glu | M-57 | 1-5 | 80.7 ± 0.3 | 18.4 ± 0.4 | 0.8 ± 0.1 | 0.05 ± 0.03 | 78.1 ± 0.5 | 20.8 ± 0.3 | 0.9 ± 0.2 | 0.09 ± 0.03 |
M-85 | 2-5 | 92.1 ± 0.2 | 7.5 ± 0.2 | 0.3 ± 0.0 | 0.06 ± 0.00 | 93.6 ± 0.1 | 6.2 ± 0.1 | 0.0 ± 0.0 | 0.09 ± 0.01 | |
f302 | 1-2 | 83.4 ± 0.2 | 16.2 ± 0.2 | 0.3 ± 0.0 | 81.2 ± 0.3 | 18.4 ± 0.1 | 0.4 ± 0.2 | |||
Gly | M-57 | 1-2 | 96.1 ± 0.0 | 3.8 ± 0.0 | 0.1 ± 0.0 | 97.2 ± 0.1 | 2.8 ± 0.1 | 0.03 ± 0.02 | ||
M-85 | 2 | 98.8 ± 0.1 | 1.1 ± 0.0 | 99.0 ± 0.0 | 0.9 ± 0.0 | |||||
Phe | M-57 | 1-9 | 85.7 ± 0.6 | 13.0 ± 0.6 | 0.6 ± 0.1 | 0.08 ± 0.03 | 86.7 ± 0.9 | 11.6 ± 0.3 | 0.5 ± 0.1 | 0.02 ± 0.01 |
f302 | 1-2 | 95.9 ± 0.3 | 4.1 ± 0.3 | 0.0 ± 0.0 | 96.7 ± 0.2 | 3.3 ± 0.2 | 0.0 ± 0.0 | |||
Ser | M-57 | 1-3 | 95.3 ± 0.3 | 4.6 ± 0.3 | 0.0 ± 0.0 | 0.07 ± 0.03 | 96.7 ± 0.1 | 3.3 ± 0.1 | 0.0 ± 0.0 | 0.09 ± 0.02 |
M-85 | 2-3 | 97.7 ± 0.1 | 2.3 ± 0.1 | 0.0 ± 0.0 | 98.0 ± 0.1 | 2.0 ± 0.1 | 0.0 ± 0.0 | |||
f302 | 1-2 | 95.6 ± 0.0 | 3.9 ± 0.0 | 0.5 ± 0.0 | 96.8 ± 0.1 | 2.8 ± 0.0 | 0.4 ± 0.0 | |||
Tyr | M-57 | 1-9 | 86.2 ± 0.7 | 12.8 ± 0.1 | 0.5 ± 0.3 | 0.06 ± 0.09 | 87.7 ± 0.2 | 11.4 ± 0.4 | 0.5 ± 0.0 | 0.08 ± 0.06 |
f302 | 1-2 | 96.1 ± 0.2 | 3.9 ± 0.2 | 0.0 ± 0.0 | 97.3 ± 0.4 | 2.7 ± 0.4 | 0.0 ± 0.0 | |||