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Table 1 Selected TBDMSa-amino acid fragments used in the study derived from D. shibae and P. gallaeciensis grown on 99% [1-13C] glucose.

From: Metabolic fluxes in the central carbon metabolism of Dinoroseobacter shibae and Phaeobacter gallaeciensis, two members of the marine Roseobacter clade

  Fragment C-Atoms Mass isotopomer distribution (% of total pool)
    D. shibae P. gallaeciensis
    M +0 M +1 M +2 M +3 M +0 M +1 M +2 M +3
Ala M-57 1-3 50.0 ± 0.2 48.2 ± 0.2 1.8 ± 0.0 0.01 ± 0.01 49.2 ± 0.0 49.3 ± 0.0 1.5 ± 0.0 0.0 ± 0.0
  M-85 2-3 96.8 ± 0.1 3.2 ± 0.1 0.0 ± 0.0   97.2 ± 0.0 2.8 ± 0.0 0.0 ± 0.0  
  f302 1-2 51.2 ± 0.1 48.2 ± 0.1 0.6 ± 0.0   50.1 ± 0.1 49.3 ± 0.1 0.6 ± 0.0  
Asp M-57 1-4 72.4 ± 0.7 23.2 ± 0.5 4.3 ± 0.2 0.12 ± 0.01 64.2 ± 0.2 29.4 ± 0.1 6.2 ± 0.2 0.13 ± 0.07
  M-85 2-4 83.3 ± 0.6 16.2 ± 0.6 0.4 ± 0.1 0.10 ± 0.03 80.0 ± 0.1 19.4 ± 0.0 0.6 ± 0.0 0.04 ± 0.02
  f302 1-2 82.1 ± 0.3 17.6 ± 0.3 0.2 ± 0.0   76.3 ± 0.1 23.5 ± 0.0 0.3 ± 0.1  
Glu M-57 1-5 80.7 ± 0.3 18.4 ± 0.4 0.8 ± 0.1 0.05 ± 0.03 78.1 ± 0.5 20.8 ± 0.3 0.9 ± 0.2 0.09 ± 0.03
  M-85 2-5 92.1 ± 0.2 7.5 ± 0.2 0.3 ± 0.0 0.06 ± 0.00 93.6 ± 0.1 6.2 ± 0.1 0.0 ± 0.0 0.09 ± 0.01
  f302 1-2 83.4 ± 0.2 16.2 ± 0.2 0.3 ± 0.0   81.2 ± 0.3 18.4 ± 0.1 0.4 ± 0.2  
Gly M-57 1-2 96.1 ± 0.0 3.8 ± 0.0 0.1 ± 0.0   97.2 ± 0.1 2.8 ± 0.1 0.03 ± 0.02  
  M-85 2 98.8 ± 0.1 1.1 ± 0.0    99.0 ± 0.0 0.9 ± 0.0   
Phe M-57 1-9 85.7 ± 0.6 13.0 ± 0.6 0.6 ± 0.1 0.08 ± 0.03 86.7 ± 0.9 11.6 ± 0.3 0.5 ± 0.1 0.02 ± 0.01
  f302 1-2 95.9 ± 0.3 4.1 ± 0.3 0.0 ± 0.0   96.7 ± 0.2 3.3 ± 0.2 0.0 ± 0.0  
Ser M-57 1-3 95.3 ± 0.3 4.6 ± 0.3 0.0 ± 0.0 0.07 ± 0.03 96.7 ± 0.1 3.3 ± 0.1 0.0 ± 0.0 0.09 ± 0.02
  M-85 2-3 97.7 ± 0.1 2.3 ± 0.1 0.0 ± 0.0   98.0 ± 0.1 2.0 ± 0.1 0.0 ± 0.0  
  f302 1-2 95.6 ± 0.0 3.9 ± 0.0 0.5 ± 0.0   96.8 ± 0.1 2.8 ± 0.0 0.4 ± 0.0  
Tyr M-57 1-9 86.2 ± 0.7 12.8 ± 0.1 0.5 ± 0.3 0.06 ± 0.09 87.7 ± 0.2 11.4 ± 0.4 0.5 ± 0.0 0.08 ± 0.06
  f302 1-2 96.1 ± 0.2 3.9 ± 0.2 0.0 ± 0.0   97.3 ± 0.4 2.7 ± 0.4 0.0 ± 0.0  
  1. atert-butyldimethylsilyl; fragmentation patterns are described elsewhere [27]