Assessment of three Resistance-Nodulation-Cell Division drug efflux transporters of Burkholderia cenocepaciain intrinsic antibiotic resistance

Table 3 Primers used in this work

Primer	Sequence (5'-3')	Restriction enzyme^a,b
KO1XL^c	GCTCTAGACTCGATGCCGCGCTGCTGGA	XbaI
KO1BL^c	TTGGATCCGCGGTTCCGACTATCGCAAG	BamHI
KO1BR^c	TTGGATCCCAGGCACGCTACCGCAG	BamHI
KO1KR^c	GGGGTACCATCTGCGTCTCTTCCTTG	KpnI
OP13LX^d	GCTCTAGAGGCGGCACACCGTCGAAC	XbaI
OP13LB^d	TTCGGGATCCCATCTGGCCGCCGTTTAT	BamHI
OP13RB^d	TTCGGGATCCTAATCGGCGATGTGTTGC	BamHI
OP13RE^d	TGGAATTCGCCACCCCCGCTCTCCCTTG	EcoRI
KO4XL^e	GCTCTAGACCGTGATCCTGAACATCGTG	XbaI
KO4NL^e	TTCATATGCGCAGACGGATCTGTACG	NdeI
KO4NR^e	TTCATATGCTGCGCGACGAGGAATGC	NdeI
KO4KR^e	GGGGTACCCTGCTGGTAACAATCTGTAA	KpnI
KO1F	GAGGTCCAGCACGATGATG	N/A
KO1R	CGAGCATGTCCGTGACCAGT	N/A
CO13OPL	TCAAAGGGGTGTGGGCGGG	N/A
CO13OPR	GATTAAGGGAATTTCTTCTTGC	N/A
KO4F	GTCGCCGCACTTCTTCTC	N/A
KO4R	TCCTTGGTACGTCTGACC	N/A

^a Restriction endonuclease sites incorporated in the oligonucleotide sequences are underlined.
^b N/A indicates the absence of restriction site.
^c The PCR cycling conditions used with these primers were: 95°C for 5 min followed by 30 cycles of 94°C for 15 s, 62°C or 52°C for 1 min respectively, and 72°C for 1 min, with a final extension at 72°C for 10 min.
^d The PCR cycling conditions used with these primers were: 95°C for 15 min followed by 25 cycles of 94°C for 1 min, 52°C for 1 min, and 72°C for 1 min, with a final extension at 72°C for 10 min.
^e The PCR cycling conditions used with these primers were: 95°C for 5 min followed by 30 cycles of 94°C for 15 s, 54°C for 1 min, and 72°C for 1 min, with a final extension at 72°C for 10 min.

ISSN: 1471-2180