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Table 2 Strains and plasmids

From: Assessment of three Resistance-Nodulation-Cell Division drug efflux transporters of Burkholderia cenocepaciain intrinsic antibiotic resistance

Strain or plasmid Relevant characteristics Source and/or reference
B. cenocepaciastrains
J2315 CF clinical isolate G. Manno
D1 J2315 ΔBCAS0591-BCAS0593 This study
D3 J2315 ΔBCAL1672-BCAL1676 This study
D4 J2315 ΔBCAL2820-BCAL2822 This study
E. coli strains
DH5α F- Φ80dlacZΔM15 Δ(lacZYA-argF)U169 endA1 recA1 hsdR17(rK- mK+) supE44 thi-1 ΔgyrA96 relA1 Laboratory stock
SY327 araD Δ(lac pro) argE(Am) recA56 nalA λ pir; Rifr [43]
pGEM-T Easy Vector for PCR cloning, Ampr Promega
pGPISce-I oriR6K, ΩTpr, mob+, containing the ISce-I restriction site [32]
pRK2013 oricolE1, RK2 derivative, Kanr, mob+, tra+ [44]
pDAISce-I pDA12 encoding the ISce-I homing endonuclease [32]
pOP1/pGPI-SceI Plasmid for construction of D1 deletion mutant This study
pOP3/pGPI-SceI Plasmid for construction of D3 deletion mutant This study
pOP4/pGPI-SceI Plasmid for construction of D4 deletion mutant This study
pSCR1 Ampr, pQF50 containing PcepI-lacZ and cepR [42]
  1. Ampr, ampicillin resistance; Kanr, kanamycin resistance; Rifr, rifampin resistance; Tetr, tetracycline resistance; Tpr, trimethoprim resistance.