Table 2 Strains and plasmids
Strain or plasmid | Relevant characteristics | Source and/or reference |
|---|---|---|
B. cenocepaciastrains | ||
J2315 | CF clinical isolate | G. Manno |
D1 | J2315 ΔBCAS0591-BCAS0593 | This study |
D3 | J2315 ΔBCAL1672-BCAL1676 | This study |
D4 | J2315 ΔBCAL2820-BCAL2822 | This study |
E. coli strains | ||
DH5α | F- Φ80dlacZΔM15 Δ(lacZYA-argF)U169 endA1 recA1 hsdR17(rK- mK+) supE44 thi-1 ΔgyrA96 relA1 | Laboratory stock |
SY327 | araD Δ(lac pro) argE(Am) recA56 nalA λ pir; Rifr | [43] |
Plasmids | ||
pGEM-T Easy | Vector for PCR cloning, Ampr | Promega |
pGPISce-I | oriR6K, ΩTpr, mob+, containing the ISce-I restriction site | [32] |
pRK2013 | oricolE1, RK2 derivative, Kanr, mob+, tra+ | [44] |
pDAISce-I | pDA12 encoding the ISce-I homing endonuclease | [32] |
pOP1/pGPI-SceI | Plasmid for construction of D1 deletion mutant | This study |
pOP3/pGPI-SceI | Plasmid for construction of D3 deletion mutant | This study |
pOP4/pGPI-SceI | Plasmid for construction of D4 deletion mutant | This study |
pSCR1 | Ampr, pQF50 containing PcepI-lacZ and cepR | [42] |