Evaluation of AHLs accumulation in the growth medium of B. cenocepacia J2315 and the D1, D3, and D4 mutant strains. C8-HSL measurement using E. coli (pSCR1) as described in Methods. C8-HSL was extracted from spent supernatants, AHL levels were measured with a volume of extract corresponding to 109 CFU. Values of AHL accumulated in the supernatant are expressed in Miller Units and in percentage in relation to the wild-type strain. The experiments were performed in triplicate giving comparable results. Significantly differences in AHL levels with respect J2315 are indicated by an * (ANOVA: P < 0.05; F 13.02; Dunnett's multiple Comparison test).