Figure 2From: Mechanism of protonophores-mediated induction of heat-shock response in Escherichia coliRate of s ynthesis of sigma-32 at different instants of cell growth. A and B represent the result of cell growth at 30°C in the presence of 50 μM CCCP, and at 50°C respectively. Pulse-label at 0, 5, 10, 15, 20, 30 minutes of cell growth and subsequent immunoprecipitation experiment using anti-sigma-32 antibody was performed as described in 'Methods'.Back to article page