Table 4 PCR and qRT-PCR primers used in this study.
Primer | Sequence (5'→3') | Description |
|---|---|---|
C42/F | CCCAAAGCTTGGGTCCTGCTCATCACCAGAAACTCCA | HindIII site, used to construct pKH11 |
CF2/R | GCTCTAGAGCAACCGCTTTCAGGCACTGTTGTTC | XbaI site, used to construct pKH11 |
Primers for RT-PCR--asterisk denotes primer for cDNA synthesis | ||
Primer | Sequence (5'→3') | Gene |
MQO RT/A* | AGGCCTGCCCGTAGACTTTC | Arth_4255 |
MQO RT/B | TCTTCACCGCCGGTATGAG | Arth_4255 |
ChrB RT/A* | CGAGGATGAGGGATCGTTTG | Arth_4254 |
ChrB RT/B | TGATCCGCAGGAACATCG | Arth_4254 |
SP RT/F | CCCGGGAGCACTTCGACTGGA | Arth_4253 |
SP RT/R* | CCTGGCGCGTTCGGTTGCAT | Arth_4253 |
Cog4RT/F | AAGGCCTACGTCTCCAACGAACA | Arth_4252 |
Cog4RT/R* | ATTCTGTCGGTGACCGTGTCAGT | Arth_4252 |
ChrAP RT/A | GCTTCATCCTGTGCTTCTTG | Arth_4251 |
ChrAP RT/B* | TGTTCATGATGCCGGTACTG | Arth_4251 |
BP RT/F | CCTGCGCCGCTACGAACTCACCGAT | Arth_4249 |
BP RT/R* | GCGCTGGTGTTCGTACAGCCCGTC' | Arth_4249 |
ChrAC RT/A* | AAGTACAGGGCCAGGTTC | Arth_4248 |
ChrAC RT/B | TGCGGTCCTGTCCTATATC' | Arth_4248 |
Lppy RT/F | AGTGACCACGGCCATCAATTTCCA | Arth_4247 |
Lppy RT/R* | TCAGGGAATGATTGTGCACGGAGA | Arth_4247 |