|
C42/F
|
CCCAAAGCTTGGGTCCTGCTCATCACCAGAAACTCCA
|
HindIII site, used to construct pKH11
|
|
CF2/R
|
GCTCTAGAGCAACCGCTTTCAGGCACTGTTGTTC
|
XbaI site, used to construct pKH11
|
|
Primers for RT-PCR--asterisk denotes primer for cDNA synthesis
|
|
Primer
|
Sequence (5'→3')
|
Gene
|
|
MQO RT/A*
|
AGGCCTGCCCGTAGACTTTC
|
Arth_4255
|
|
MQO RT/B
|
TCTTCACCGCCGGTATGAG
|
Arth_4255
|
|
ChrB RT/A*
|
CGAGGATGAGGGATCGTTTG
|
Arth_4254
|
|
ChrB RT/B
|
TGATCCGCAGGAACATCG
|
Arth_4254
|
|
SP RT/F
|
CCCGGGAGCACTTCGACTGGA
|
Arth_4253
|
|
SP RT/R*
|
CCTGGCGCGTTCGGTTGCAT
|
Arth_4253
|
|
Cog4RT/F
|
AAGGCCTACGTCTCCAACGAACA
|
Arth_4252
|
|
Cog4RT/R*
|
ATTCTGTCGGTGACCGTGTCAGT
|
Arth_4252
|
|
ChrAP RT/A
|
GCTTCATCCTGTGCTTCTTG
|
Arth_4251
|
|
ChrAP RT/B*
|
TGTTCATGATGCCGGTACTG
|
Arth_4251
|
|
BP RT/F
|
CCTGCGCCGCTACGAACTCACCGAT
|
Arth_4249
|
|
BP RT/R*
|
GCGCTGGTGTTCGTACAGCCCGTC'
|
Arth_4249
|
|
ChrAC RT/A*
|
AAGTACAGGGCCAGGTTC
|
Arth_4248
|
|
ChrAC RT/B
|
TGCGGTCCTGTCCTATATC'
|
Arth_4248
|
|
Lppy RT/F
|
AGTGACCACGGCCATCAATTTCCA
|
Arth_4247
|
|
Lppy RT/R*
|
TCAGGGAATGATTGTGCACGGAGA
|
Arth_4247
|
