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Table 3 Bacterial strains and plasmids used in this study.

From: High-level chromate resistance in Arthrobactersp. strain FB24 requires previously uncharacterized accessory genes

Strain or plasmid Description Reference
Arthrobacter   
   FB24 CrR [6]
   D11 CrS derivative of FB24 This work
E. coli   
   JM110 dam - dcm - Stratagene
Plasmids   
pAOWA10128 7.3 kb insert in pMCL200 obtained from DOE-JGI. Contains Arth_4248-Arth_4254. DOE-JGI
pBluescript II SK+ 3.0 kb, ApR, lacZ, used for sublconing inserts prior to ligation into pART2. Promega
pART2 4.6 kb, KmR, pCG100 ori, ColE1 ori, vector for expression in Arthrobacter [55]
pKH11 10.6 kb PCR product from FB24 plasmid 3 (CP000457) containing Arth_4247-4255 in pBluescript II SK+ This worka
pKH12 Insert from pKH11 cloned into pART2 This work
pKH21 7.3 kb insert from pAOWA10128 in pBluescript II SK+ This work
pKH22 Insert from pKH21 cloned into pART2 This work
pKH32 3.7 kb EcoRI-KpnI fragment from pKH21 cloned into pART2. Contains Arth_4248-4249. This work
pKH42 3.8 kb XhoI-BglII fragment from pKH21 cloned into pART2. Contains Arth_4251-Arth_4254. This work
pKH52 8.3 kb insert from MluI-BglII digest of pKH11 to delete Arth_4252 and Arth_4252 cloned into pART2 This work
pKH62 pKH22 digested with SfiI to delete Arth_4249-Arth_4252. This work
pKH72 pKH12 digested with ScaI and XbaI to delete Arth_4247. This work
  1. aA schematic of each construct is presented in Figure 3.