Table 10 Oligonucleotide primers used in the real-time PCR
Gene | Forward primer | Reverse primer |
|---|---|---|
dmsA | ATGTTGCCGGACAAGCACAAGATG | TCTCAATGGACAACGGCTACCACA |
dmsB | AACAGGCATCGATTGCACCGTTAC | ACTTGGACGTGCGTGTTTATTGGC |
napB | GCGCATGGCAACCTAAACATTGGT | TACAGGCTTTGCAGTAGCGGAAAC |
napD | TCGGCTAAAGCAAGCTGTCTGTCA | TAGCGCAAGTGAAAGCGGACATTC |
napF | ACAACCGTCTCCGCAACTTCTACA | TTGGCTACAACGGAAGAAGCATGG |
ilvH | GAAAGTTTAACCGTTGCGCCGACT | ACGTTCAATATGCTCGGTAGGGCT |
pgaA | GGGAACCGGTGTGAATGCAATGAA | TGTTGGAACGTTTGTGAAGACGCC |
pgaC | ATCGTTGCGTTACACCAAGCGAAC | ACCGACATACTTGCCTCTTGCGAT |
apxIVA | TTGGACTTCACCTGCAAACATGCC | CGGGCAAATATTCCAAAGCGCAGA |
relA | TCGGACAGTTGAAGTGGGAAT | TGCAAGGCGATTACTCGGTAA |
syp | AAGAAACGCCGAATGATGCACAGG | ACACCTCGATAGCACCACCTTTGT |
lamB | CTGCTAAAGAGAGTTTACCGATGCCA | TGCAACATTACGGGCAGGTAAACG |
malK | GCGTGTTGCAATTGGACGTACCTT | CATGGCTTCGATTTGGTCATGCGT |
malM | AGCGACACCGTCAAAGACAGAACT | CCAACGTTTGGCTAAATGTGCGGA |
malT | TCCTTGATGAGCTTTCGACCCACA | TAAACCGAGCACCTGCCATTCTCT |
malP | ACGCTTAGCCGCCTGCTATTTAGA | CACGCATCGCCTTCTTCATGTTGT |
malQ | ATGCCTATCGGCCTTTACCGTGAT | ACCGACAGAGGCATCTAGCACAAA |
malE | AACCGATGAAGGACTCACAACCGT | TTTCCGCATTCGCCATAGTTGCTG |
malF | TGCCGTTAATGATTGCCAGCTTCG | GCAGCCGCTAAACCAAAGTCTTGT |
malG | AGTGTTACTCATGCGGACGGAAGT | GCATACGCAGCAGTGGTTGAAAGT |