malT knockout mutation invokes a stringent type gene-expression profile in Actinobacillus pleuropneumoniae in bronchoalveolar fluid

Table 1 Differential expression of maltose-regulon genes in BALF-exposed A. pleuropneumoniae CM5

Gene	Putative function	ΔΔC_T ± SD	Fold-change*
malE (T)	Periplasmic maltose binding protein	-2.82 ± 0.51	7.06 (4.95-10.05)
malE (R)		0 ± 0.84	1 (0.55-1.79)
malF (T)	Intrinsic membrane protein of maltose transport system	-2.79 ± 1.01	6.91 (3.43-13.92)
malF (R)		0 ± 0.39	1 (0.76-1.31)
malG (T)	Intrinsic membrane protein of the maltose transport system	-2.6 ± 0.40	6.06 (8-4.59)
malG (R)		0 ± 0.40	1(0.76-1.31)
malK (T)	ATP-binding protein of the maltodextrin transporter	-1.10 ± 0.39	2.14 (1.6-2.8)
malK (R)		0 ± 0.76	1(0.59-1.69)
lamB (T)	Maltoporin	-1.73 ± 0.46	3.31 (2.41-4.56)
lamB (R)		0 ± 0.35	1(0.78-1.27)
malP (T)	Maltodextrin phosphorylase	-0.85 ± 0.46	1.80(1.31-2.46)
malP (R)		0 ± 0.79	1(0.58-1.72)
malQ (T)	Amylomaltase	-0.96 ± 0.48	1.94(1.39-2.71)
malQ (R)		0 ± 0.55	1(0.68-1.46)
malT (T)	Transcriptional activator of maltose-regulon genes	-0.75 ± 0.32	1.68(1.34-2.09)
malT (R)		0 ± 0.79	1(0.58-1.72)

* Fold change is the fold increase or decrease in the level of expression of a gene in the wild type exposed to BALF (target sample, abbreviated as T) relative to the level of expression of the gene in the wild type exposed to BHI (calibrator or reference sample, abbreviated as R), as measured by real-time PCR.
Values in the parentheses represent the range in the fold change.

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