Qualitative and quantitative detection of the oomycete A. astaci. A: Diagnostic qPCR/MCA primers (blue arrows) target A. astaci-specific sites in the homologous chitinase genes CHI1, CHI2 and CHI3, but not homologous sequences of related oomycetes and fungi. Parentheses contain GenBank accession numbers. Dots and letters represent identical and substituted nucleotides compared to the A. astaci sequence, respectively. B: Qualititative detection of A. astaci by qPCR/MCA. The left and right peaks are derived from amplification of the endogenous control, and the chitinase genes CHI2 &CHI3, respectively. Red plot: A. astaci, blue plot: A. frigidophilus. C: Quantitative detection of A. astaci by TaqMan qPCR. The standard curve of the assay demonstrates quantification down to 25 copies.