Relative levels of DhAHP transcript of three yeasts and their DhAHP overexpression transformants. Cells of D. hansenii (A), S. cerevisiae (B) and P. methanolica (C) were grown in media containing 3.5, 2.0 and 2.5 M NaCl, respectively, in the presence or absence of methanol for 72 min, and their DhAHP transcripts determined by real-time RT-PCR. For each species, the level for the wild type strain grown in media without methanol was taken as 1. Since the wild type strains of S.c. and P.m do not contain DhAHP their DhAHP transcript levels were low while their overexpression transformants showed high levels of expression relatively. Data presented were means +/- S.D. from 3–4 replicates of measurement.