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Figure 4 | BMC Microbiology

Figure 4

From: Characterization of a salt-induced DhAHP, a gene coding for alkyl hydroperoxide reductase, from the extremely halophilic yeast Debaryomyces hansenii

Figure 4

A. Southern blot showing a single restriction fragment of D. hansenii. Approximately 20 μg total DNA was digested to completion with EcoRI (lane 1) or BamHI (lane 2), electrophoresed on agarose gel, transferred to nylon membrane and hybridized to DhAHP probe. B. Northern blot of DhAHP transcript as affected by salt treatment. Total RNA was isolated and electrophoresed on agarose-formaldehyde gel, transferred to nylon membrane and hybridized to DhAHP probe (A). The gel was stained with ethidium bromide prior to blotting (B). Lane 1 and 2 indicate RNAs extracted from D. hansenii cells after inducted by 2.5 M NaCl for 0 and 16 min, respectively.

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BMC Microbiology

ISSN: 1471-2180

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