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Table 1 Bacteria and plasmids used in this study

From: Extracellular secretion of Carocin S1 in Pectobacterium carotovorum subsp. carotovorumoccurs via the type III secretion system integral to the bacterial flagellum

Bacterium or plasmid Relevant characteristics Source
E. coli
   1830 pro-met-KmrNmr, containing transposon Tn5 on the ?sucidal? plasmid pJB4JI Gantotti et al. [37]
   DH5 supE44hsdR17recA1endA1gyrA1thi-1relA1 Hanahan and Reusch et al [26, 38]
Pectobacterium carotovorum subsp. carotovorum
   89-H-4 putative biocontrol agent Laboratory stock
   H-rif-8-6 89-H-4, Rifr this work
   Ea1068 wild type Laboratory stock
   T-29 wild type Laboratory stock
   E108 wild type Laboratory stock
   A-100 wild type Laboratory stock
   86-H-2 wild type Laboratory stock
   TH12-2 H-rif-8-2, flhC:: Tn5, Rifr, Kanr this work
   KH17 H-rif-8-2, flh D::Kan, Rifr, Kanr this work
   FliC-KO H-rif-8-2, fli C::Kam, Rifr, Kamr this work
   FlhA-KO H-rif-8-2, flh A::Kam, Rifr, Kamr this work
plasmid
   pBR322 Ampr, Kanr Bolivar et al [39]
   pBYL2DC Ampr, flhDC this work
   pBYL2C Ampr, flhC this work
   pBYL2D Ampr, flhD this work
   pBFC Ampr, fliC this work
   pBFA Ampr, flhA this work
  1. Ampr indicates ampcillin resistance, Rifr indicates rifampicin resistance, and Kanr indicates Kanamycin resistance.