Skip to main content

Table 1 Oligonucleotide primers used in this study

From: Direct and negative regulation of the sycO-ypkA-ypoJ operon by cyclic AMP receptor protein (CRP) in Yersinia pestis

Target gene

Primer sequence (5'→3')

EMSA (Sense/antisense)

 

   sycO

ATATTCTGGGACGGGTTT/TTCCTGCTGAGTTTCTGC

   YPO1099

AGCCCTCTCTCCCTAGCC/GCAGTTGCCAGACCGC

   YPO0180

GCTACCGAGCCTAACCC/AGGCACCCATCTCATGG

Real-time PCR or RT-PCR (Sense/antisense)

 

   sycO

GCCCTTGTTTCGCTTGGAGTG/AGTTCCTGCTGAGTTTCTGCTG

   ypkA

GCTAAGATTGAACGCTCCATTG/TCAGAACAACGCCAACCATC

   yopJ

AATCCAGGCGAACAATAAATATCC/CACTGAAATGTATTCCACCTTCC

   sycO-ypkA intergenic

CAGGAACTGCCCCTTCATAC/ATACCGTTTTCCTCCGATATTGAG

   ypkA-yopJ intergenic

TGCGAGAGCTGACGACCATC/TCATTACTGATTAAAGAACTGGTC

   lacA

CCGATAACGATTGGCAATAACG/GCGAATAACCCGACAAGGAAC

   16s rRNA

TTACCTACTCTTGACATCCAC/GCTGGCAACAAAGGATAAG

DNase I footprinting (Sense/antisense)

 

   sycO

CAGATTTGTCTACAGGTTCG/CTCAGCATAATAACGACTCGG

LacZ reporter fusion (Sense/antisense)

 

   sycO

GCGGAATTCAGGAACGGGAAGATTTAC/GCGGGATCCAATCTCTCTGCATGAACG

Primer extension

 

   sycO

CTCAGCATAATAACGACTCGG

Back to article page

BMC Microbiology

ISSN: 1471-2180

Contact us