Figure 3From: Direct and negative regulation of the sycO-ypkA-ypoJ operon by cyclic AMP receptor protein (CRP) in Yersinia pestisElectrophoretic mobility shift assay. The band of DNA fragment containing the promoter region of sycO disappeared with increasing amounts of CRP protein, and a retarded DNA band with decreased mobility turned up (Fig. 3a), which presumably represented the CRP-DNA complex. But for YPO0180 and YPO1099, the CRP-DNA complex did not appear even His-CRP was increased to 4 μg for each reaction mixture (Fig. 3b).Back to article page