Figure 6

Effect of IAA on sporulation in wild type and cgopt1 -silenced mutants. Strains were cultured on EMS plates with and without IAA. Spores were collected and counted after 5 days. A. Plates were kept in continuous light (left) or darkness (right). White bars – wild type, Black bars – Ori51, Gray bars – Ori83. B. Fungi were cultured in the dark on EMS (left) or EMS with 500 μM IAA (right). C. Fungi were cultured in the dark on EMS plates with increasing IAA concentrations. Bars are the mean and SD of five replications. Differences between wild type and the mutants were found significant according to t-test (P < 0.05) in the following treatments: sporulation in the light (A), sporulation in dark on EMS with 500 μM IAA (B, C), sporulation in the dark on EMS with 250 μM (C). Repetition of experiments led to similar results.